PReP-Bicyc：邻近驱动区域选择性肽双环化用于噬菌体展示库
美国德州农工大学团队报道了PReP-Bicyc，一种无需添加剂、与噬菌体兼容的肽库位点选择性双环化策略。该方法使用理性设计的连接子4,6-dichloropyrimidine-2-carbonitrile（4,6-DCCPm），先与N端半胱氨酸经腈介导形成噻唑啉，再通过邻近驱动对两个内部半胱氨酸进行分子内硫醇芳基化，在温和条件下高效成环并保持噬菌体活性。
%20Microsoft%20Corporation.%20Permission%20is%20hereby%20granted,%20free%20of%20charge,%20to%20any%20person%20obtaining%20a%20copy%20of%20this%20software%20and%20associated%20documentation%20files%20(the%20'Software'),%20to%20deal%20in%20the%20Software%20without%20restriction,%20including%20without%20limitation%20the%20rights%20to%20use,%20copy,%20modify,%20merge,%20publish,%20distribute,%20sublicense,%20and/or%20sell%20copies%20of%20the%20Software,%20and%20to%20permit%20persons%20to%20whom%20the%20Software%20is%20furnished%20to%20do%20so,%20subject%20to%20the%20following%20conditions:%20The%20above%20copyright%20notice%20and%20this%20permission%20notice%20shall%20be%20included%20in%20all%20copies%20or%20substantial%20portions%20of%20the%20Software.%20THE%20SOFTWARE%20IS%20PROVIDED%20'AS%20IS',%20WITHOUT%20WARRANTY%20OF%20ANY%20KIND,%20EXPRESS%20OR%20IMPLIED,%20INCLUDING%20BUT%20NOT%20LIMITED%20TO%20THE%20WARRANTIES%20OF%20MERCHANTABILITY,%20FITNESS%20FOR%20A%20PARTICULAR%20PURPOSE%20AND%20NONINFRINGEMENT.%20IN%20NO%20EVENT%20SHALL%20THE%20AUTHORS%20OR%20COPYRIGHT%20HOLDERS%20BE%20LIABLE%20FOR%20ANY%20CLAIM,%20DAMAGES%20OR%20OTHER%20LIABILITY,%20WHETHER%20IN%20AN%20ACTION%20OF%20CONTRACT,%20TORT%20OR%20OTHERWISE,%20ARISING%20FROM,%20OUT%20OF%20OR%20IN%20CONNECTION%20WITH%20THE%20SOFTWARE%20OR%20THE%20USE%20OR%20OTHER%20DEALINGS%20IN%20THE%20SOFTWARE%20--%3e%3csvg%20width='32'%20height='32'%20viewBox='0%200%2032%2032'%20fill='none'%20xmlns='http://www.w3.org/2000/svg'%3e%3cpath%20d='M12.3136%2012.7574L20.4999%2011L25.7487%2013.4646L11.6065%2027.6067C9.6539%2029.5593%206.48807%2029.5593%204.53545%2027.6067C2.58283%2025.6541%202.58283%2022.4882%204.53545%2020.5356L5.59212%2019.479L6.64879%2019.5664V18.4223L9.84605%2015.225L10.8949%2015.1953V14.1762L12.3136%2012.7574Z'%20fill='%23C3EF3C'/%3e%3cpath%20d='M21.8596%202.50428C21.2738%201.91849%2020.3241%201.91849%2019.7383%202.50428C19.1525%203.09007%2019.1525%204.03982%2019.7383%204.6256L20.0918%204.97916L15.1174%209.95364L15.5%2011L14.1038%2010.9672L12.3136%2012.7574L25.7486%2013.4645L27.1629%2012.0502L27.5165%2012.4038C28.1023%2012.9896%2029.052%2012.9896%2029.6378%2012.4038C30.2236%2011.818%2030.2236%2010.8682%2029.6378%2010.2825L21.8596%202.50428Z'%20fill='%23AEDDFF'/%3e%3cpath%20d='M15.1421%209.92896L17.4402%2012.2271C17.7331%2012.5199%2017.7331%2012.9948%2017.4402%2013.2877C17.1473%2013.5806%2016.6724%2013.5806%2016.3795%2013.2877L14.0814%2010.9896L15.1421%209.92896Z'%20fill='%23008463'/%3e%3cpath%20d='M10.8994%2014.1716L13.1975%2016.4697C13.4904%2016.7626%2013.4904%2017.2375%2013.1975%2017.5304C12.9046%2017.8233%2012.4297%2017.8233%2012.1368%2017.5304L9.83875%2015.2323L10.8994%2014.1716Z'%20fill='%23008463'/%3e%3cpath%20d='M8.95495%2020.7123L6.65685%2018.4142L5.59619%2019.4748L7.89429%2021.7729C8.18718%2022.0658%208.66206%2022.0658%208.95495%2021.7729C9.24784%2021.48%209.24784%2021.0052%208.95495%2020.7123Z'%20fill='%23008463'/%3e%3c/svg%3e){kind=small}
%20Microsoft%20Corporation.%20Permission%20is%20hereby%20granted,%20free%20of%20charge,%20to%20any%20person%20obtaining%20a%20copy%20of%20this%20software%20and%20associated%20documentation%20files%20(the%20'Software'),%20to%20deal%20in%20the%20Software%20without%20restriction,%20including%20without%20limitation%20the%20rights%20to%20use,%20copy,%20modify,%20merge,%20publish,%20distribute,%20sublicense,%20and/or%20sell%20copies%20of%20the%20Software,%20and%20to%20permit%20persons%20to%20whom%20the%20Software%20is%20furnished%20to%20do%20so,%20subject%20to%20the%20following%20conditions:%20The%20above%20copyright%20notice%20and%20this%20permission%20notice%20shall%20be%20included%20in%20all%20copies%20or%20substantial%20portions%20of%20the%20Software.%20THE%20SOFTWARE%20IS%20PROVIDED%20'AS%20IS',%20WITHOUT%20WARRANTY%20OF%20ANY%20KIND,%20EXPRESS%20OR%20IMPLIED,%20INCLUDING%20BUT%20NOT%20LIMITED%20TO%20THE%20WARRANTIES%20OF%20MERCHANTABILITY,%20FITNESS%20FOR%20A%20PARTICULAR%20PURPOSE%20AND%20NONINFRINGEMENT.%20IN%20NO%20EVENT%20SHALL%20THE%20AUTHORS%20OR%20COPYRIGHT%20HOLDERS%20BE%20LIABLE%20FOR%20ANY%20CLAIM,%20DAMAGES%20OR%20OTHER%20LIABILITY,%20WHETHER%20IN%20AN%20ACTION%20OF%20CONTRACT,%20TORT%20OR%20OTHERWISE,%20ARISING%20FROM,%20OUT%20OF%20OR%20IN%20CONNECTION%20WITH%20THE%20SOFTWARE%20OR%20THE%20USE%20OR%20OTHER%20DEALINGS%20IN%20THE%20SOFTWARE%20--%3e%3csvg%20width='32'%20height='32'%20viewBox='0%200%2032%2032'%20fill='none'%20xmlns='http://www.w3.org/2000/svg'%3e%3cpath%20d='M4.17488%2028.0659L3.93406%2027.8251C1.35531%2025.2464%201.35531%2021.022%203.93406%2018.4333L6.27291%2016.0944L14.0776%2014.4555L16.0978%206.26952L18.4333%203.93406C21.012%201.35531%2025.2363%201.35531%2027.8251%203.93406L28.0659%204.17488C30.6447%206.75363%2030.6447%2010.978%2028.0659%2013.5667L25.7355%2015.8972L17.8981%2017.93L15.9157%2025.717L13.5667%2028.0659C10.988%2030.6447%206.75363%2030.6447%204.17488%2028.0659Z'%20fill='%2326C9FC'/%3e%3cpath%20d='M22.5%206C22.7761%206%2023%205.77614%2023%205.5C23%205.22386%2022.7761%205%2022.5%205C22.2239%205%2022%205.22386%2022%205.5C22%205.77614%2022.2239%206%2022.5%206Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M20.5%208C20.7761%208%2021%207.77614%2021%207.5C21%207.22386%2020.7761%207%2020.5%207C20.2239%207%2020%207.22386%2020%207.5C20%207.77614%2020.2239%208%2020.5%208Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M22.5%2010C22.7761%2010%2023%209.77614%2023%209.5C23%209.22386%2022.7761%209%2022.5%209C22.2239%209%2022%209.22386%2022%209.5C22%209.77614%2022.2239%2010%2022.5%2010Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M25%2011.5C25%2011.7761%2024.7761%2012%2024.5%2012C24.2239%2012%2024%2011.7761%2024%2011.5C24%2011.2239%2024.2239%2011%2024.5%2011C24.7761%2011%2025%2011.2239%2025%2011.5Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M25%207.5C25%207.77614%2024.7761%208%2024.5%208C24.2239%208%2024%207.77614%2024%207.5C24%207.22386%2024.2239%207%2024.5%207C24.7761%207%2025%207.22386%2025%207.5Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M26.5%2010C26.7761%2010%2027%209.77614%2027%209.5C27%209.22386%2026.7761%209%2026.5%209C26.2239%209%2026%209.22386%2026%209.5C26%209.77614%2026.2239%2010%2026.5%2010Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M6%2022.5C6%2022.7761%205.77614%2023%205.5%2023C5.22386%2023%205%2022.7761%205%2022.5C5%2022.2239%205.22386%2022%205.5%2022C5.77614%2022%206%2022.2239%206%2022.5Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M7.5%2025C7.77614%2025%208%2024.7761%208%2024.5C8%2024.2239%207.77614%2024%207.5%2024C7.22386%2024%207%2024.2239%207%2024.5C7%2024.7761%207.22386%2025%207.5%2025Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M10%2026.5C10%2026.7761%209.77614%2027%209.5%2027C9.22386%2027%209%2026.7761%209%2026.5C9%2026.2239%209.22386%2026%209.5%2026C9.77614%2026%2010%2026.2239%2010%2026.5Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M7.5%2021C7.77614%2021%208%2020.7761%208%2020.5C8%2020.2239%207.77614%2020%207.5%2020C7.22386%2020%207%2020.2239%207%2020.5C7%2020.7761%207.22386%2021%207.5%2021Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M10%2022.5C10%2022.7761%209.77614%2023%209.5%2023C9.22386%2023%209%2022.7761%209%2022.5C9%2022.2239%209.22386%2022%209.5%2022C9.77614%2022%2010%2022.2239%2010%2022.5Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M11.5%2025C11.7761%2025%2012%2024.7761%2012%2024.5C12%2024.2239%2011.7761%2024%2011.5%2024C11.2239%2024%2011%2024.2239%2011%2024.5C11%2024.7761%2011.2239%2025%2011.5%2025Z'%20fill='%2300A6ED'/%3e%3cpath%20d='M25.7548%2015.8553L16.1382%206.23865L6.25833%2016.1185L15.875%2025.7352L25.7548%2015.8553Z'%20fill='%2300A6ED'/%3e%3c/svg%3e){kind=small}
%20Microsoft%20Corporation.%20Permission%20is%20hereby%20granted,%20free%20of%20charge,%20to%20any%20person%20obtaining%20a%20copy%20of%20this%20software%20and%20associated%20documentation%20files%20(the%20'Software'),%20to%20deal%20in%20the%20Software%20without%20restriction,%20including%20without%20limitation%20the%20rights%20to%20use,%20copy,%20modify,%20merge,%20publish,%20distribute,%20sublicense,%20and/or%20sell%20copies%20of%20the%20Software,%20and%20to%20permit%20persons%20to%20whom%20the%20Software%20is%20furnished%20to%20do%20so,%20subject%20to%20the%20following%20conditions:%20The%20above%20copyright%20notice%20and%20this%20permission%20notice%20shall%20be%20included%20in%20all%20copies%20or%20substantial%20portions%20of%20the%20Software.%20THE%20SOFTWARE%20IS%20PROVIDED%20'AS%20IS',%20WITHOUT%20WARRANTY%20OF%20ANY%20KIND,%20EXPRESS%20OR%20IMPLIED,%20INCLUDING%20BUT%20NOT%20LIMITED%20TO%20THE%20WARRANTIES%20OF%20MERCHANTABILITY,%20FITNESS%20FOR%20A%20PARTICULAR%20PURPOSE%20AND%20NONINFRINGEMENT.%20IN%20NO%20EVENT%20SHALL%20THE%20AUTHORS%20OR%20COPYRIGHT%20HOLDERS%20BE%20LIABLE%20FOR%20ANY%20CLAIM,%20DAMAGES%20OR%20OTHER%20LIABILITY,%20WHETHER%20IN%20AN%20ACTION%20OF%20CONTRACT,%20TORT%20OR%20OTHERWISE,%20ARISING%20FROM,%20OUT%20OF%20OR%20IN%20CONNECTION%20WITH%20THE%20SOFTWARE%20OR%20THE%20USE%20OR%20OTHER%20DEALINGS%20IN%20THE%20SOFTWARE%20--%3e%3csvg%20width='32'%20height='32'%20viewBox='0%200%2032%2032'%20fill='none'%20xmlns='http://www.w3.org/2000/svg'%3e%3cpath%20d='M20.3432%202.29289C20.1556%202.10536%2019.9013%202%2019.6361%202H7C5.89543%202%205%202.89543%205%204V28C5%2029.1046%205.89543%2030%207%2030H25C26.1046%2030%2027%2029.1046%2027%2028V9.36394C27%209.09872%2026.8946%208.84437%2026.7071%208.65683L20.3432%202.29289Z'%20fill='%23B4ACBC'/%3e%3cpath%20d='M19.6821%203.00001L7%203C6.44772%203%206%203.44772%206%204V28C6%2028.5523%206.44772%2029%207%2029L25%2029C25.5523%2029%2026%2028.5523%2026%2028V9.45333L19.6821%203.00001Z'%20fill='%23F3EEF8'/%3e%3cpath%20d='M9.5%2012H22.5C22.7761%2012%2023%2012.2239%2023%2012.5C23%2012.7761%2022.7761%2013%2022.5%2013H9.5C9.22386%2013%209%2012.7761%209%2012.5C9%2012.2239%209.22386%2012%209.5%2012ZM9.5%2015C9.22386%2015%209%2015.2239%209%2015.5C9%2015.7761%209.22386%2016%209.5%2016H22.5C22.7761%2016%2023%2015.7761%2023%2015.5C23%2015.2239%2022.7761%2015%2022.5%2015H9.5ZM9%2018.5C9%2018.2239%209.22386%2018%209.5%2018H22.5C22.7761%2018%2023%2018.2239%2023%2018.5C23%2018.7761%2022.7761%2019%2022.5%2019H9.5C9.22386%2019%209%2018.7761%209%2018.5ZM9.5%2021C9.22386%2021%209%2021.2239%209%2021.5C9%2021.7761%209.22386%2022%209.5%2022H17.5C17.7761%2022%2018%2021.7761%2018%2021.5C18%2021.2239%2017.7761%2021%2017.5%2021H9.5Z'%20fill='%23998EA4'/%3e%3cpath%20d='M26%209.45325H21.3892C20.4464%209.45325%2019.6821%208.68896%2019.6821%207.74614V3L26%209.45325Z'%20fill='%23CDC4D6'/%3e%3c/svg%3e){kind=small}
推荐理由：原文给出一种无需添加剂的噬菌体兼容双环化策略，并展示其对PD-1配体筛选的可用性。